I was deeply impressed by the presentation of Dr. Femke de Vrij from Erasmus MC on “Human adherent cortical organoids to study neuropsychiatric disorders” during the CONNECT symposium 2025. This groundbreaking research introduces a simplified method for generating long-term human-induced pluripotent stem cell (hiPSC)-derived adherent cortical organoids with reproducible sizes and the potential for high-throughput screening in a 384-well format.
Dr. de Vrij’s lab developed a platform where hiPSC-derived neural progenitor cells (NPCs) are seeded into 384-well plates. Within eight weeks, these NPCs differentiate into self-organized, layered radial structures containing multiple neural subtypes, astrocytes, and oligodendrocytes. These organoids can be cultured long-term and exhibit features such as dendritic spines, axonal myelination, and robust neuronal activity.
A standout advantage of these adherent cortical organoids is the avoidance of necrotic cores—a frequent issue with free-floating organoids. This innovation makes them particularly suited for high-throughput drug discovery, neurotoxicological screening, and studying pathophysiological mechanisms in brain disorders.
New Concepts in Microfluidic Chip Design
Inspired by this remarkable research and a great publication, I have conceptualized three new designs for microfluidic chips that connect adherent cortical organoids to a blood-brain barrier (BBB) model. These designs promise to open exciting new avenues for advanced research.
Single BBB Connection
This chip design enables the connection of a single adherent organoid to a BBB channel. It allows researchers to study detailed interactions between a cortical brain organoid and the blood-brain barrier.
Two Organoids in Fluid Communication
This concept connects two adherent organoids via a BBB channel, facilitating the analysis of fluid exchange and signaling between the structures. It is especially valuable for studying neuronal communication and systemic effects.
Assembloids for Cancer Research
The third design facilitates direct contact between two adherent organoids to create an "assembloid." This model enables the study of complex mechanisms such as cancer metastasis via the perivascular space without extravasation.
The Future of Neuroscience
The integration of these new concepts of PimCell microfluidic chips with open-top chambers and 384-well plate compatibility for co-culturing adherent cortical organoids offers unprecedented opportunities for advancing neuroscience. From unraveling neurological disease processes to crafting targeted therapies, these technologies provide an invaluable platform for research.
With these innovations, the potential impact on both science and practical applications is enormous. These breakthroughs pave the way for personalized medicine, novel drug development, and a deeper understanding of the human brain’s intricate weaknesses.
The combination of adherent cortical organoids in a multiwell format with advanced microfluidic chips marks a revolutionary step forward in neuroscience. Together, these technologies open doors to exciting discoveries and transformative applications.
At PimCell®, we specialize in microfluidic devices tailored for advanced 3D cell cultures. Our microfluidic concepts enhances in-vitro organ models, ensuring optimal conditions for brain organoids. It’s an ideal solution for researchers exploring organ-on-a-chip technology in neuroscience.
Ready to elevate your research? PimCell’s organ-on-a-chip solutions are designed to empower your studies in drug screening platforms, tissue engineering, and more. Whether you're a researcher at a university, a biotech startup, or a pharmaceutical company, our microfluidic chips provide the precision and reliability you need.
👉 Contact us now to learn more about how PimCell® can help you revolutionize your research with brain assembloids and organ-on-a-chip technology.
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